《植物生理学报》 2015, 51(4): 465-470

蛇足石杉的离体培养

马英姿*, 刘江海, 许欢, 刘芬

中南林业科技大学生命科学与技术学院, 长沙410004

通信作者：马英姿；E-mail: ma_yingzi@163.com；Tel: 0731-85623494

摘　要：

本研究以蛇足石杉活体顶芽为外植体, 研究了其表面及内生菌灭菌方法、外植体的增殖和生根方法。结果表明, 表面灭菌后再采用0.5 mg•L^-1孔雀石绿及100 mg•L^-1的AAS联合灭菌, 可获取较好的外植体灭菌效果。灭菌后的外植体仍带有一种内生真菌, 但外植体可与该内生菌共生培养, 并获得了蛇足石杉的增殖侧芽, 侧芽极易从主茎分离, 且生根率较顶芽高, 30 d生根率可达90%, 培养的根也呈二叉分枝状。

关键词：蛇足石杉; 离体培养; 内生真菌; 共生

收稿：2014-11-25   修定：2015-03-12

资助：湖南省教育厅科学研究重点项目(2012A0146)和湖南省科技厅科技计划项目(2013TZ2024)。

In Vitro Culture of Huperzia serrata

MA Ying-Zi*, LIU Jiang-Hai, XU Huan, LIU Fen

College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China

Corresponding author: MA Ying-Zi; E-mail: ma_yingzi@163.com; Tel: 0731-85623494

Abstract:

In this study, the terminal buds of Huperzia serrata were used as explants, to study the surface and endophytic bacteria sterilization, the explant proliferation and rooting method. The results showed that after the surface sterilization, the combined sterilization of 0.5 mg•L^-1 malachite green and 100 mg•L^-1 AAS could obtain good effect on sterilization of explant. After sterilized, the explants still contained an endophytic fungus, but could symbiosis culture with the endophytic fungus. And the proliferation buds of H. serrata were obtained, these buds were separated from the stem easily, had a higher rooting rate than that of terminal buds. The rooting rate reached 90% after 30 d, and the root were two forks branch.

Key words: Huperzia serrata; culture in vitro; endophyte; symbiotic